Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKALITGITGQDGSYLAEFLLEKGYEVHGITRRASISNTAHIDH-------LMGKITLHDGDLSDSSSLIRIISIVQPDEIYNLAAQSHVQVSFDVPEYSGDVDALGVLRILEACRILGLTKKTKVYQASTSELYGKVEEVPQRETTPFHPYSPYAVAKQYGFWITKEYREAYGMFAVNGILFNHESERRGENFVTRKITLAAGRIAEGLQDHLELGNMDSLRDWGYAKDYVECMWMILQHETPEDFVIATGEQHTVRDFTEKAFAANGITIRWEGTGIDEKGYDVETGKMLVCVN--PQWFRPTDVDNLWGDPTKAKTVLGWNPQKTSYEELVSIMAKHDRELAKREAALKKASDLIE 5UZH Chain:A ((1-342)) SKVALITGITGQDGSYLAEFLLEKGYMVYGIIRRSSSFNTGRVEHLYKDIHITKAKFKLLYGDLTDTGNLISIIAKIKPDEIYNLAAQSHVKVSFEMPEYTANVDGIGTLRLLEAIRACGLEKKTKFYQASTSELYGLVQEVPQKETTPFYPRSPYACAKLYSYWIVVNYREAYNMFALNGILFNHESIRRGPTFVTRKITMAVARIKLGLQDCLYLGNLDAERDWGHAKDYVEAMWLMLQQEQPRDFCVATGEKHSVREFVEKAFACIGQTVEWKGER--GTVEEHGVVDGVVRVRVDPRYFRPTEVDQLLGDPTLAETVLGWKRKVS-FEELVRGMVEGDIEL---------------

General information: TITO was launched using: RESULT: Template: 5UZH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1883 15183 8.06 45.59 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.85 3D Compatibility (PKB) : 8.06 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.376

(partial model without unconserved sides chains): PDB file : Tito_5UZH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5UZH-query.scw PDB file : Tito_Scwrl_5UZH.pdb: