Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNAIPTLTPLAMPCNSSFEAKDWEILSSHWYPVARIQDVSTA-PQRVKLLDVNMALYKTESGEIHLVRDICPHRGVPLTKGWVD--GEEIVCPYHGLRYNT-EGKCTQIPAQPELTKISDRFSLTKFPVVQRYGLIWTSIHGRDIAKANIPVLDTWDDAEHQAILPPFVDIGGSSGRQLEGFIDVAHFAWVHHNAFANRDNPIVPKY----------HTERTNYGLK---TVYISNV-SNYPHELKHL----EP---EG-FLWKRTFEVYPPFSAVLTVDFPEDGILKILNACCPISSNKTRLFVPLTRNFDQTG--------DLEKVYAFNAQIFA-EDQDMVESQKPEELPLD-LMMEAHFEADRSSTTYRRILAEWGLSKRYTV 3VMH Chain:C ((24-376)) ------------------------GFRNHWYPVMFSKEINEGEPKTLKLLGENLLVNRI-DGKLYCLKDRCLHRGVQLSVKVECKTKSTITCWYHAWTYRWEDGVLCDILTNPTSA-QIGRQKLKTYPVQEAKGCVFIYLGDGDPPPLARDTPPNFLDDDMEIL-GKNQIIKSNWRLAVENGFDPSHI-YIHKDSILVKDNDLALPLGFAPGGDRKQQTRVVDDDVVGRKGVYDLIGEHGVPVFEGTIGGEVVREGAYGEKIVANDISIWLPGVLKVNPF-PN-PDMMQFEWYVPIDENTHYYFQTLGKPCANDEERKKYEQEFESKWKPMALEGFNNDDIWAREAMVDFYADDKGWVNEILFESDEAIVAWRKLASEHNQGI----

General information: TITO was launched using: RESULT: Template: 3VMH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1669 37077 22.21 116.96 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain C : 0.65 3D Compatibility (PKB) : 22.21 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.389

(partial model without unconserved sides chains): PDB file : Tito_3VMH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3VMH-query.scw PDB file : Tito_Scwrl_3VMH.pdb: