Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLIFLPGASGSTTFWHPLIEKLPQQYRAKIIGYPSFGDTPESL----EVKSFEDLTNYVVNQ---I-HDESVIIAQSMGGIFAVTAALQKPQLVKGLVLIATSGGINLEP--F-N---V--------------QDWREAYR-----QA-FLKYPDW----F--------IT-----TNANYEEFLSDINIKTLLIWGDNDPVSPVQVGQYLNQKFENSTLYVVKGGDHQLAEKYADEVAVQIKNYLKGLM 1WOM Chain:A ((21-270)) ASIMFAPGFGCDQSVWNAVAPAFEEDHRVILFDYVGSGHSDLRAYDLNRYQTLDGYAQDVLDVCEALDLKETVFVGHSVGALIGMLASIRRPELFSHLVMVGPSPCYLNDPPEYYGGFEEEQLLGLLEMMEKNYIGWATVFAATVLNQPDRPEIKEELESRFCSTDPVIARQFAKAAFFSDHREDLSKVTVPSLILQCADDIIAPATVGKYMHQHLPYSSLKQMEARGHCPHMSHPDETIQLIGDYLKAH-

General information: TITO was launched using: RESULT: Template: 1WOM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 890 -41189 -46.28 -206.98 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -46.28 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.466

(partial model without unconserved sides chains): PDB file : Tito_1WOM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1WOM-query.scw PDB file : Tito_Scwrl_1WOM.pdb: