Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIDLYYWGTPNGHKITIALEEMGLDYTLYPINILENDQFQPDFLKISPNNKIPAIVDQDG---PRGEPISVFESGAILQYLGRKTGL----FYPTDEQERVEVEQWLMWQMGGLGPMLGQNHHFNRFAPEKIPYAIDRYVNETKRLYGVLNKQLI-----RQKFVAGE-YSIADMAILPWILRYEWQGIQLEDYPYVQEYIVRLTARPAVQKALSIKVI 4IVF Chain:D ((7-220)) PIKLYTAPTPNGYKISIFLEVLGLDYEVQKFDLSKNETKEDWFVKLNPNGRIPTINDPNFKGVDGG--LVLSQTGAILQYLADTYDKEHKFSYPAGTAEYYKTLEYLIFQVAENGPIQGQANHFVFAAKEKVPYGINRYITDTKRIYGVFEDILSRNKANDSKYLVGDRYTVADFALLGWAYRLSRLEIDINQWPLLGKWYDSLLKLPAVQKGFEV---

General information: TITO was launched using: RESULT: Template: 4IVF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 914 -56076 -61.35 -278.99 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain D : 0.80 3D Compatibility (PKB) : -61.35 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.476

(partial model without unconserved sides chains): PDB file : Tito_4IVF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IVF-query.scw PDB file : Tito_Scwrl_4IVF.pdb: