Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKLSIGLIIMLSLFGCTDSK---AKNQEKFLTHIHNNTPEPYKECMVMYIKNHWDDVWKTYDSEKVREARGETDIVNFMIERFLPECKK 1WO4 Chain:A ((1-25)) ------------AVSACALSKCAAAANVAAHMTHCAK-----------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1WO4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 43 -4718 -109.72 -214.45 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -109.72 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.535

(partial model without unconserved sides chains): PDB file : Tito_1WO4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1WO4-query.scw PDB file : Tito_Scwrl_1WO4.pdb: