Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQETKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEIGAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDAKYAEYLAEL-DKEKPVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPERYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL 5J8N Chain:A ((4-257)) YNLISWNVNGLRAAVK---------KGFLDLLLEHRFDIVCVQETKVSQDKLPRE-VKN----IQGYYNYFVSA---EQNGYSGVGTFSKNKP-IKLEKGM-GI-EVFDREGRFLRTDYEDFVLLNIYFPNGKMSQERLGYKMAFYDAFLDYANALKSEGKKLVICGDVNTAHKEIDLARPKQNEMISGFLPEERAWMDKFLAAGYLDSFRMFNPE-GGNYSWWSYRTGA-RSRNVGWRLDYVFVSENLRENVKSASIYPEIMGSDHCPVGLELEF

General information: TITO was launched using: RESULT: Template: 5J8N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1414 -86914 -61.47 -343.53 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -61.47 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.471

(partial model without unconserved sides chains): PDB file : Tito_5J8N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5J8N-query.scw PDB file : Tito_Scwrl_5J8N.pdb: