Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGLGGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFKLLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTIVPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLRGAHTSTTKFDPEVFLAVDCSPAGDVY-GGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTTAEEAGIKYQYYCG--KGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQAFLQALVKKLDRSTVDLIKHY 1VHO Chain:A ((5-335)) -METGKLLMELSNLDGPSGYETNVVSYIKSVIEPFVDEAKTTRHGSLIGYKKGKG--IGKLAFFAHVDEIGFVVSKV-EGQFARLEPV--------YASKVRIYTKNG-IERGVIGMLAPHLQDSESRKKVPTYDEIFVDLSL------CERGVRVGDIAVIDQTAFE-T-NGKVVGKALDNRASCGVLVKVLEFLKRYDHPWDVYVVFSVQEETGCLGALTGAYEINPDAAIVMDVTFASEPPFSDHIELGKGPVIGL---GPVVDRNLVQKIIEIAKKHNVSLQEEAVGGR----TDFVQLVRNGVRTSLISIPLKYMHTPVEMVDPRDVEELARLLSLVAVELE----------

General information: TITO was launched using: RESULT: Template: 1VHO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1822 -126001 -69.16 -398.74 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -69.16 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.445

(partial model without unconserved sides chains): PDB file : Tito_1VHO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VHO-query.scw PDB file : Tito_Scwrl_1VHO.pdb: