Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQLTLVRHGEAAPPVN-GNDIKRPLTARGHAQAEQTATFLKDIV-KPNIFVVSPLLRAQETLAHIQTYFKDVPVLLCDKIKPDDDAKEAIEWLSQI-PYESIVVVCHMNVVGHIAELLTHET------FNPFALAEARIYDQAVIANGLSTQKNSFIPTI 2RFL Chain:H ((9-169)) TRVYLLRHAKAAWAAPGERDFDRGLNEAGFAEAEIIADLAADRRYRPDLILSSTAARCRQTTQAWQRAF--IDIVYIDEMYN-ARSETYLSLIAAQTEVQSVMLVGHNPTMEATLEAMIGEDLLHAALPSGFPTSGLAVLDQD---KNRWRLIDFLAP--

General information: TITO was launched using: RESULT: Template: 2RFL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 684 1035 1.51 7.23 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain H : 0.71 3D Compatibility (PKB) : 1.51 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.523

(partial model without unconserved sides chains): PDB file : Tito_2RFL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2RFL-query.scw PDB file : Tito_Scwrl_2RFL.pdb: