Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTDIQLNLIGRTQELFVEDIQHWNNKLIEIVSSSKFLVLGGAGSIGQAVVKEIFKRNPKKLHVVDISENNLTEVVRDIRSSFGYIDGDFQTFALDIGSAEYDAYIENDGDFDYVLNLSALKHVRSEKDPFTLMRMIHVNILNTEKTIQQSIAKGVKKYFCVSTDKAA----------------NPVNMMGGSKRIMEMFLMRQSQ--YIDISTARFANVAFSDGS------LLHGFNQRIQKNQPIAAP--NDIRRYFVTPKESGELCLMSCLLGENRDIFFPKLSEHLHLITFADIAVKYLNNLGYEPYLCESEDEARSLIQTLPKQGKWPCLFAGSTTTGEKDFEEFFTDNETLDMQRFNNLGVIKNELNIEEDKLQVFEEKINSMLLSKHWNKEEIVDLFNYMMPNFGHKETGLYLDGKM 4ZRM Chain:B ((3-261)) ---------------------------------HMNILVTGGAGFIGSHVVDKLIENG-YGVIVVDNLSSGKVE---NLN-------RNALFYEQSIEDEEMMERIFSLHRPEYVFHLAAQASVAISVR--EPARDAKTNIIGSLVLLEKSIKYGVKKFIFSSTGGAIYGENVKVFPTPETEIPHPISPYGIAKYSTEMYLEFFAREYGLKYTVLRYANVYGPRQDPYGEAGVVAIFTERMLRGEEVHIFGDGEYVRDYVYVDDVVRANLLAMEKG-DNEVFNIGTGRGT---TVNQLFKLLKEITGYD-------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4ZRM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1222 -68721 -56.24 -294.94 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain B : 0.62 3D Compatibility (PKB) : -56.24 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.430

(partial model without unconserved sides chains): PDB file : Tito_4ZRM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZRM-query.scw PDB file : Tito_Scwrl_4ZRM.pdb: