Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQPVRVAVTGAAGQIGYSLLFRIASGEMLGKDQPVILQLLEVPVEKAQQALKGVMMELDDCAFPLLAGMIGTDDPKVAFKDADYALLVGSRPRGPGMERADLLKVNGEIFIGQGQALNEV-ASRDVKVLVVGNPANTNAYIAMKSAPDLPAKNFTAMLRLDHNRALTQVAQKAGVVVADIEKLTVWGNHSPTMYADYRFATANGESLKDKINDPAWNKDVFLPTVGKRGAAIIEARGLSSAASAANAAIDHMRDWALGTN-GKWVTMGVPSDG-SYGIPEGVMFGFPVTT-ENGEYKIVQGLEIDEFSRERINFTLNELEEERAAIADMVK 1CIV Chain:A ((39-367)) WKKIINVAVSGAAGMISNHLLFKLASGEVFGPDQPISLKLLGSER--SFAALEGVAMELEDSLYPLLRQVSIGIDPYEIFQDAEWALLIGAKPRGPGMERADLLDINGQIFAEQGKALNAVAS-PNVKVMVVGNPCNTNALICLKNAPNIPPKNFHALTRLDENRAKCQLALKAGVFYDKVSNVTIWGNHSTTQVPDFLNAKIHGIPVTEVIRDRKWLEDEFTNMVQTRGGVLIKKWGRSSAASTAVSIVDAIRSLVTPTPEGDWFSTGVYTNGNPYGIAEDIVFSMPCRSKGDGDYEFVKDVIFDDYLSKKIKKSEDELLAEKKCVAHLTG

General information: TITO was launched using: RESULT: Template: 1CIV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1850 -104315 -56.39 -320.97 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : -56.39 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.554

(partial model without unconserved sides chains): PDB file : Tito_1CIV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1CIV-query.scw PDB file : Tito_Scwrl_1CIV.pdb: