Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSCYLITGGAGFIGSTLANHLG-EEHVVIVVDDLSMGKVENLDMTKNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMILELIRKYQPDLKRIVFSSSAA-VYGDEPT-LPKKEESVIRPLTPYAVDKFAAEQYVLDYCHLYDVPGSAVRFFNVYGPNQNPN---SPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSRDFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEERSGDIHDSLADITKIQS-IGYQPKYGILSGMTNYLKTEIN 4ZRN Chain:A ((4-308)) -MNILVTGGAGFIGSHVVDKLIENGYGVIVVDNLSSGKVENL--NRNALFYEQSIEDEEMMERIFSLHRPEYVFHLAAQASVAISVREPARDAKTNIIGSLVLLEKSIKYG--VKKFIFSSTGGAIYGENVKVFPTPETEIPHPISPYGIAKYSTEMYLEFFAREYGLKYTVLRYANVYGPRQDPYGEAGVVAI--------FTERML-RGEEVHIFGDGEYVRDYVYVDDVVRANLLAMEKG--DNEVFNIGTGRGTTVNQLFKLLKEITGYDKEPVYKPPRKGDVRKSILDYTKAKEKLGWEPKVSLEEGLKLTVEYFR-

General information: TITO was launched using: RESULT: Template: 4ZRN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1735 -11321 -6.53 -37.99 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -6.53 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.478

(partial model without unconserved sides chains): PDB file : Tito_4ZRN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZRN-query.scw PDB file : Tito_Scwrl_4ZRN.pdb: