Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFPTPEHVKQAGIAAIE-EDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGATEAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSD----------TGFVCQPEQFERAFEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGT-HMSIATY--LPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQKAAAEAL-IN------GVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAI------QT---DSWQFCVELAEQAKVACIPGIAFGPE-----GEGYIRISYASSMEKLHEACKRIKDFLTEQ 3E2Y Chain:B ((2-410)) --------AKRIEGLDSNVWVEFTK-LAADPSVVNLGQGFPDISPPSYVKEELSKAAFIDNMNQYTRGFGHPALVKALSCLYGKIYQRQIDPNEEILVAVGAYGSLFNSIQGLVDPGDEVIIMVPFYDCYEPMVRMAGAVPVFIPLRSKPTDGMKWTSSDWTFDPRELESKF---SSKTKAIILNTPHNPLGKVYTRQELQVIADLCVKHDTLCISDEVYEWLVYTGHTHVKIATLPGMWERTITIGSAGKTFSVTGWKLGWSIGPAHLIKHLQTVQQNSFYTCATPLQAALAEAFWIDIKRMDDPECYFNSLPKELEVKRDRMVRLLNSVGLKPIVPDGGYFIIADVSSLGADLSDMNSDEPYDYKFVKWMTKHKKLTAIPVSAFCDSKSKPHFEKLVRFCFIKKDSTLDAAEEIFRAWN---

General information: TITO was launched using: RESULT: Template: 3E2Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1993 -46999 -23.58 -125.66 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -23.58 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.553

(partial model without unconserved sides chains): PDB file : Tito_3E2Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3E2Y-query.scw PDB file : Tito_Scwrl_3E2Y.pdb: