Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WFFRSQGRKEAERQLLAPINKAGSFLIRESETNKGAFSLSVKDV-TTQGELIKHYKIRCLDEGGYYISPRITFPSLQALVQHY 1NZL Chain:A ((4-86)) WYFGKITRRESERLLLNPENPRGTFLVRESET--GAYCLSVSDFDNAKGLNVKHYKIRKLDSGGFYITSRTQFSSLQQLVAYY

General information: TITO was launched using: RESULT: Template: 1NZL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 280 -16213 -57.90 -202.66 target 2D structure prediction score : 0.79 Monomeric hydrophicity matching model chain A : 0.85 3D Compatibility (PKB) : -57.90 2D Compatibility (Sec. Struct. Predict.) : 0.79 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.678

(partial model without unconserved sides chains): PDB file : Tito_1NZL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1NZL-query.scw PDB file : Tito_Scwrl_1NZL.pdb: