Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------CFYTVSRKEATEMLQKNPSLGNMILRPGSDSR--NYSITIRQEIDIP-RIKHYKVMSVGQNYTIELEKPVTLPNLFSVIDYF--------------------- 2PLD Chain:A ((1-105)) GSPGIHESKEWYHASLTRAQAEHMLMRVPRDGAFLVRKRN--EPNSYAISFRAE----GKIKHCRVQQEG-QTVMLGN--SEFDSLVDLISYYEKHPLYRKMKLRYPINEENSS

General information: TITO was launched using: RESULT: Template: 2PLD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 181 6663 36.81 95.19 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 36.81 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.470

(partial model without unconserved sides chains): PDB file : Tito_2PLD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2PLD-query.scw PDB file : Tito_Scwrl_2PLD.pdb: