Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYYTLEEKEVFMREALREAEIALE--HDEIPIGCVIVKDGEIIGRGHNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCV------MCSGAIGLARIPNVVYGAKNQKFGAAGSLYDILTDERLNHRVEVETGILEDECAAIMQDFFRNRRKK 3ZPG Chain:A ((23-170)) --SELKQDQYWMQQAIELAKRGLYSTKPNPNVGCVIVKDDQLIGEGFHPK----AGQPHAEVFALRQAGEQ-----AQGATAYVTLEPCAHYGRTPPCAEALVKAQVKKVVVACPDPNPLVAGKGVQILK----NAGIEVEIGICEDLAAKLNQGFLKAMS--

General information: TITO was launched using: RESULT: Template: 3ZPG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 770 -13875 -18.02 -100.54 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -18.02 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.581

(partial model without unconserved sides chains): PDB file : Tito_3ZPG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZPG-query.scw PDB file : Tito_Scwrl_3ZPG.pdb: