Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTDIQLNLIGRTQELFVEDIQHWNNKLIEIVSSSKFLVLGGAGSIGQAVVKEIFKRNPKKLHVVDISENNLTEVVRDIRSSFGYIDGDFQTFALDIGSAEYDAYIENDGDFDYVLNLSALKHVRSEKDPFTLMRMIHVNILNTEKTIQQSIAKGVKKYFCVSTDKAA----------------NPVNMMGGSKRIMEMFLMRQSQ--YIDISTARFANVAFSDGS------LLHGFNQRIQKNQPIAAP--NDIRRYFVTPKESGELCLMSCLLGENRDIFFPKLSEHLHLITFADIAVKYLNNLGYEPYLCESEDEARSLIQTLPKQGKWPCLFAGSTTTGEKDFEEFFTDNETLDMQRFNNLGVIKNELNIEEDKLQVFEEKINSMLLSKHWNKEEIVDLFNYMMPNFGHKETGLYLDGKM 4ZRN Chain:A ((4-261)) ----------------------------------MNILVTGGAGFIGSHVVDKLIENG-YGVIVVDNLSSGKVE---NLN-------RNALFYEQSIEDEEMMERIFSLHRPEYVFHLAAQASVAISVR--EPARDAKTNIIGSLVLLEKSIKYGVKKFIFSSTGGAIYGENVKVFPTPETEIPHPISPYGIAKYSTEMYLEFFAREYGLKYTVLRYANVYGPRQDPYGEAGVVAIFTERMLRGEEVHIFGDGEYVRDYVYVDDVVRANLLAMEKG-DNEVFNIGTGRGT---TVNQLFKLLKEITGYD-------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4ZRN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1180 -65142 -55.20 -280.78 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -55.20 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.449

(partial model without unconserved sides chains): PDB file : Tito_4ZRN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZRN-query.scw PDB file : Tito_Scwrl_4ZRN.pdb: