Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------------MSKSIEKFPKEL----------VSPIAQLHSLVEKNSKLHIKELFAAEQDRFQNYSVKFD-------QLVFDYSKHRITKSVLEQLFALAKTKQLTHWIERLFSQDKVNCTEQRAAMHWALRLPSEYSKF---PELTKQVHTQLQRMYVLVEKIHAGQYRGATGEVIQDVVNIGVGGSDLGPQMVTHALCDFKVKTAKPLNVHFVSTMDGSQLSDLLHQLRPETTLFIISSKSFGTIDTLSNAQTVRQWLEKALGKHDR----VVKSHFIGVSTKAEKMTEWGIAPENQLLLWDWVGGRYSLWSCIGFPIALTIGIDGFQQLLAGAHAVDEHFQNTNFEQNIPVLMALLGIWNNNFLNIQTHAVLPYDGRLKYFAAYLQQLEMESNGKSVQRDGQKVELDTCPIVWGEVGPNAQHAFYQLLHQGTQAVSCDFIAPIQ---RYNADHFTYVENAEALIEQHHLALSNCLAQSRLLAFGNEALDSAELKNLPIYKQYEGNQPSSTLLLKELNPYSLGMLIALYEHKVFVQSVIWNINPFDQWGVEKGKQIADQLLPILNGAQNDLSALDASTRGLIKILLGKVDG 4QFH Chain:A ((6-615)) HHHMKDQYLKDLTVHLNESNAAPANTSMAVASFNMPHEITRRMRPLGVDADTSLTSCPSWRRLQELYEIHGSESILKNFDECKDRFQRYSLEVDLRSSDKNFVFLDYSKTHINDEIKDVLFKLVEERGIRAFMRALFAGEKVNTAENRSVLHIALRNRSNRPIFVNGHDVMPLVNKVLEQMKKLSEKVRRGEWKGQSGKPIRHVVNIGIGGSDLGPMMACEALRPF---SDRRISMHFVSNIDGTHLSEVLNLVDLESTLFIIASKTFTTQETITNALSARNEFLKFLSSRGISEAGAVAKHFVALSTNAEKVKEFGIDEENMFQFWDWVGGRYSLWSAIGLSVMISIGYDNFVELLTGAHIMDEHFINAPTENNLPIILALVGIWYNNFFGSETQAILPYDQYLWRLPAYLQQLDMESNGKGATKNGRMVSTHTGPIIFGEAGTNGQHAFYQLIHQGTKLIPCDFIGAIQTQNYIGEHHRILMSNFFAQTEALMIGKTPEEVKRELESAGGK--SEDEIQLLIPQKTFTGGRPSNSLLVKALTPRALGAIIAMYEHKVLVQGAIWGINSYDQWGVELGKLLAKSILLQLQPGQK-VTNHDSSTNGLIELFNERSH-

General information: TITO was launched using: RESULT: Template: 4QFH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2770 61535 22.21 112.08 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : 22.21 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.505

(partial model without unconserved sides chains): PDB file : Tito_4QFH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QFH-query.scw PDB file : Tito_Scwrl_4QFH.pdb: