Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAESTTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEYAPKAVENF--MTHAKDG--------YYNGLTFHRVIKDFMIQGGD-PKGDGTGGESIWGEGFETEINNHLYNIRGALSMARSQDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGMDVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK 2IGW Chain:A ((18-165)) ------------------------------------------------------------------------------GRIVMELYDDVVPKTAGNFRALCTGENGIGKSGKPLHFKGSKFHRIIPNFMIQGGDFTRGNGTGGESIYGEKFPDENFKEKHTGPGVLSMANA-GPNTNGSQFFLCT---------VKTEW---------------LDGKHVVFGRVVEGLDVVKAVES-NGSQSGKPVKDCMI------------

General information: TITO was launched using: RESULT: Template: 2IGW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 671 29443 43.88 214.91 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 43.88 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.459

(partial model without unconserved sides chains): PDB file : Tito_2IGW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2IGW-query.scw PDB file : Tito_Scwrl_2IGW.pdb: