Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPELPEVETVRKGLERLVVGKTIQKVQVLWPK-IIEQPETPIFEASLVGETIQSIGRRGKFLIFHLD-HCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDGSQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKPLLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGGTTIRSYLNALGESGGFQVALHVYQQTGKPCIR--CGTPIVKTKVAQRGTHYCPNCQRLKIAK 3TWL Chain:A ((2-281)) -PELPEVEAARRAIEENCLGKKIKRVIIADDNKVIHGISPSDFQTSILGKTIISARRKGKNLWLELDSPPFPSFQFGMAGAIYIK----WPSKYSKFFVELDDGLELSFTDKRRFAKVRLLANPT--SVSPISELGPDALLEPMTVDEFAESLAKKKITIKPLLLDQGYISGIGNWIADEVLYQARIHPLQTASSLSKEQCEALHTSIKEVIEKAVEVDADSS-----------QFPSNWIFHNREKKPGKAFVDGKKIDFITA------YVPELQKLYGK-

General information: TITO was launched using: RESULT: Template: 3TWL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1347 37131 27.57 146.76 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : 27.57 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.380

(partial model without unconserved sides chains): PDB file : Tito_3TWL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TWL-query.scw PDB file : Tito_Scwrl_3TWL.pdb: