Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNLNQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKRTPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSAYAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFIVGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP 1AHH Chain:A ((2-249)) ---FNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDINADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPKPF-DMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYASSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPE-------IEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGG---------

General information: TITO was launched using: RESULT: Template: 1AHH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1354 -151635 -111.99 -611.43 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -111.99 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.474

(partial model without unconserved sides chains): PDB file : Tito_1AHH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1AHH-query.scw PDB file : Tito_Scwrl_1AHH.pdb: