Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKALITGITGQDGSYLAEFLLEKGYEVHGITRRASISNTAHIDH--LMGKITLHDGDLSDSSSLIRIISIVQPDEIYNLAAQSHVQVSFDVPEYSGDVDALGVLRILEACRILGLTKKTKVYQASTSELYGKVEEVPQRETTPFHPYSPYAVAKQYGFWITKEYREAYGMFAVNGILFNHESERRGENFVTRKITLAAGRIAEGLQDHLELGNMDSLRDWGYAKDYVECMWMILQHETPEDFVIATGEQHTVRDFTEKAFAANGITIRWEGTGIDEKGYDVETGKMLVCVNPQWFRPTDVDNLWGDPTKAKTVLGWNPQKTSYEELVSIMAKHDRELAKREAALKKASDLIE 2Z1M Chain:A ((3-337)) GKRALITGIRGQDGAYLAKLLLEKGYEVYGADRRSGEFASWRLKELGIENDVKIIHMDLLEFSNIIRTIEKVQPDEVYNLAAQSFVGVSFEQPILTAEVDAIGVLRILEALRTVK--PDTKFYQASTSEMFGKVQEIPQTEKTPFYPRSPYAVAKLFGHWITVNYREAYNMFACSGILFNHESPLRGIEFVTRKITYSLARIKYGLQDKLVLGNLNAKRDWGYAPEYVEAMWLMMQQPEPDDYVIATGETHTVREFVEKAAKIAGFDIEWVGEGINEKGIDRNTGKVIVEVSEEFFRPAEVDILVGNPEKAMKKLGWKPRTT-FDELVEIMMEADLKR---------------

General information: TITO was launched using: RESULT: Template: 2Z1M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1991 27045 13.58 81.22 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : 13.58 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.380

(partial model without unconserved sides chains): PDB file : Tito_2Z1M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Z1M-query.scw PDB file : Tito_Scwrl_2Z1M.pdb: