Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MINLGII-GTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGSHY--EGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFS-TPKEMDEIIHLANEHNVYF-FEA-ARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQVLDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFG--MPKEVHYFP--RKISTGVDGLGWGILRYENFDVSIQPGKIGDSYL-PSEIYFDSGTLVMNGV---NAIEQAQFHDRAHKEIIDLAVTAAENPMADEAEDFANVMAH-PKDPAWGLLYEEW----IELARNVNQVIYDLRKNGGIKFDADNEEDF 1YDW Chain:B ((6-360)) QIRIGV-MGCADIARKVSRAIHLAPNATISGVASRSLEKAKAFATANNYPESTKIHGSYESLLEDPEIDALYVPLPTSLHVEWAIKAAEKGKHILLEKP-VAMNVTEFDKIVDACEANGVQIMDGTMWV--HNPRTALLKEFLSDSERFGQLKTVQSCFSFAGDEDFLKNDIRVKPGLDGLGALGDAGWYAIRATLLANNFELPKTVTAFPGAVLNEAGVILSCGASLSWEDGRTATIYCSFLANLTMEITAIGTKGTLRVHDFIIPYKETEASFTTSTKAWFNDLVTAWVSPPSEHTVKTELPQEACMVREFAIKNNGAKPDGYWPSISRKTQLVVDAVKESVDKNYQQISLS--

General information: TITO was launched using: RESULT: Template: 1YDW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1831 24639 13.46 74.44 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : 13.46 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.291

(partial model without unconserved sides chains): PDB file : Tito_1YDW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1YDW-query.scw PDB file : Tito_Scwrl_1YDW.pdb: