Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTDTALIMALPNESKGLFE-QA--------------------GIEVHYSGIGKINAAFKAFEVIQKTGCKTLINLGTAGSSS--FNRHDLVEIKTFVQRDMDVSPLGFEVGVTP-----LDDHLAAEIHLQT---HFADLPKGICGTGDSFETGQPKV--------ACDVVDMEGYALAKVCHKLGVRLISVKYITDGADDTAHLDWEENLLLGAQKLLALYQNHF 4QEZ Chain:A ((2-226)) ---RIAVIGAMEEEVRILRDKLEQAETETVAGCEFTKGQLAGHEVILLKSGIGKVNAAMSTTILLERYKPEKVINTGSAGGFHHSLNVGDVVISTEVRHHDVDVTAFNYEYGQVPGMPPGFKADEALVALAEKCMQ----VVKGMIATGDSFMSDPNRVAAIRDKFENLYAVEMEAAAVAQVCHQYEVPFVIIRALSDIAGKESNVSFDQFLDQAALHSTNFIVKV-

General information: TITO was launched using: RESULT: Template: 4QEZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 889 11462 12.89 63.68 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : 12.89 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.445

(partial model without unconserved sides chains): PDB file : Tito_4QEZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QEZ-query.scw PDB file : Tito_Scwrl_4QEZ.pdb: