Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MATAVNCNICFIGGGNMAQALIGGLISRG-LPPTRITVSDPVEQIRQL--LQEKEVHVTQDNTAAIQNADVVVLAVKPQVLANVLRPLKGLLSDK-LVISIIAGAEI----KTISNLIDSERIVRVMPNTPALVQTGAHGIYATDVVG-ASDRELTSQILAATGLTIWVNSEAQIDAVTAVSGSGPAYFFYLMESMIRAGKNLGLDEKVATALTLQTALGAAQMAITSSNTPSELRKNVTSPNGTTQAALEVFDRAQISQNIQSALAVAQKRSQELAQELSDSAK 5UAU Chain:D ((24-295)) -------SVGFIGAGQLAFALAKGFTAAGVLAAHKIMASSPDMDLATVSALRKMGVKLTPHNKETVQHSDVLFLAVKPHIIPFILDEIGADIEDRHIVVSCAAGVTISSIEKKLSAFRPAPRVIRCMTNTPVVVREGA-TVYATGTHAQVEDGRLMEQLLSSVGFCTEVE-EDLIDAVTGLSGSGPAYAFTALDALADGGVKMGLPRRLAVRLGAQALLGAAKMLLHSEQHPGQLKDNVSSPGGATIHALHVLESGGFRSLLINAVEASCIRTREL-QSMAD---

General information: TITO was launched using: RESULT: Template: 5UAU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1184 -33372 -28.19 -126.89 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain D : 0.80 3D Compatibility (PKB) : -28.19 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.516

(partial model without unconserved sides chains): PDB file : Tito_5UAU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5UAU-query.scw PDB file : Tito_Scwrl_5UAU.pdb: