Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQLTLVRHGEAAPPVNGNDIKRPLTARGHAQAEQTATFLKDIV-KPNIFVVSPLLRAQETLAHIQTYFKDVPVLLCDKIKPDDDAKEAIEWLSQI--PYESIVVVCHMNVVGHIAELLTHET---------------FNPFALAEARIYDQAV----IANGLSTQKNSFIPTI 4HBZ Chain:A ((20-184)) RTLVLMRHAAAGSAV--RDHDRPLTPDGVRAATAAGQWLRGHLPAVDVVVCSTAARTRQTLAATGIS---AQVRYRDELYGG-GVDEILAEVAAVPADASTVLVVGHAPTIPATGWELVRQSLLNRDADPSSGAGDELRHFAAGTFAVLSTTGAWADLAQAGAELQLVQHP--

General information: TITO was launched using: RESULT: Template: 4HBZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 703 -38749 -55.12 -270.97 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -55.12 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.395

(partial model without unconserved sides chains): PDB file : Tito_4HBZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HBZ-query.scw PDB file : Tito_Scwrl_4HBZ.pdb: