Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTETTIQGLAASLNPARAARKIIGFVPTMGNLHEGHLTLVREAKKL-CDVVVVSIFVNPTQFGPGEDFDNYPRTLEQDSRLLADVGCDIIFAPSVEQMYGTQPRLTNISVSQITDDLCGSSRPGHFDGVALVVTKLFNIVQPNYAFFGQKDYQQLAVIRQFVQDLNIPLEVIGVPIVRAEDGLALSSRNGYLTPEQRQVAPVIYQGLKQAEEQLHQGKDLQQVLADLKTLLTDNGFVVDYVEARQPNLLAASQFDRDIVLFVAAKLGGTRLIDNLQVAFTPQ 3COY Chain:B ((32-286)) -------------------GRRVM-LVPTMGALHEGHLALVRAAKRVPGSVVVVSIFVNPMQ--------AYPRTPDDDLAQLRAEGVEIAFTPTTAAMYPDGLR-TTVQPGPLAAELEGGPRPTHFAGVLTVVLKLLQIVRPDRVFFGEKDYQQLVLIRQLVADFNLDVAVVGVPTVREADGLAMSSRNRYLDPAQRAAA-VALSAALTAAAHAATAGAQAALDAARAVLDAAPGVAVDYLELRDIG-LGPMPLNGSGRLLVAARLGTTRLLDNIAI-----

General information: TITO was launched using: RESULT: Template: 3COY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1249 -61658 -49.37 -250.64 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -49.37 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.574

(partial model without unconserved sides chains): PDB file : Tito_3COY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3COY-query.scw PDB file : Tito_Scwrl_3COY.pdb: