Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSGSTITPWVVGNWKMNPMRANANQLIEEFKQ--LLQQNQIADENCHVGVAPVSIALTT--VQAQLQDAARTVYTVAQDVSRVAGTGAYTGEVSAELLKDSQINFVLVGHSERRDIFGDNVEILKAKLQNALNAGMTVIYCVGESLEQREQGQAEQVVLQQICDIAPVVTAEQWQNQVVIAYEPIWAIGTGKTASPQDAQAMHAKIREGLCQLTPA--GSNIAILYGGSVKAENAVELAACPDINGALVGGASLNAASFYQIVQAFAQSK 5EYW Chain:B ((8-247)) --------FVGGNWKMNGDKAAIDGIISFMKTGPLSPNTEVV-----VGCPQCYLMYTREHMPANIGIAAQNCYKVAK--------GAFTGEISPAMVKDCGCEWVILGHSERRNVFGEPDQLISEKVGHALEAGLKVIPCIGEKLEDREGNRTQEVVFAQMKALLPNIS--DW-SRVVLAYEPVWAIGTGKTASPEQAQEVHADLRQWLRDNVNAEVAESTRIIYGGSVSAGNCQELAKKGDIDGFLVGGAALK-PDFVQIINA-----

General information: TITO was launched using: RESULT: Template: 5EYW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1264 -39332 -31.12 -168.09 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.82 3D Compatibility (PKB) : -31.12 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.558

(partial model without unconserved sides chains): PDB file : Tito_5EYW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EYW-query.scw PDB file : Tito_Scwrl_5EYW.pdb: