Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYHGMEQPEYLLSHQAMVNEPDKFYQFVKKIY-HPEAKPNIIHLEIAHLAKE-KNVWVVSQNIDGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEY------LHSDKHQACGG-QIRPDIVLYGEGFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQP-SAKILVINQTPVYLAE-PYTFIQEDGAVVFKKIQELGADYD 1S7G Chain:A ((2-248)) --EDEIRKAAEILAKSKHAVVFTGAGISAESGIPTFRGEDGLWRKYD-PEEVASISGFKRNPRAFWEFSMEMKDKLFAEPNPAHYAIAELERMGIVKAVITQNIDMLHQRAGSRRVLELHGSMDKLDCLDCHETYDWSEFVEDFNKGEIPRCRKCGSYYVKPRVVLFGEPLPQRTLFEAIEEAKHCDAFMVVGSSLVVYPAAELPYIAKKAGAKMIIVNAEPTMADPIFDVKIIGKAGEVLPKIVEEVKR--

General information: TITO was launched using: RESULT: Template: 1S7G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1188 -32684 -27.51 -138.49 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -27.51 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.431

(partial model without unconserved sides chains): PDB file : Tito_1S7G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1S7G-query.scw PDB file : Tito_Scwrl_1S7G.pdb: