Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVSKATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRY-LNRV---NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLILYFQAMVEGISKPEI---YAKLLPYTAEWTEVLIKGIAPKKK 3EUP Chain:B ((4-203)) --KELSKSDRTRQFIIESTAPVFNV-KGLAGTSLTDLTEATNLTKGSIYGNFENKEAVAIAAFDYNWGHVKSVLTAKVQACNTYKEMLLVYSSMYNDADGSLFPVGGCPLLNTTIEA-DDTHDALRKKA-GEAILSWKKNLVTIIKKGIQAKEFRPDTDVTKIAFSMIALVEGAILIHRATKNRAYSDYVFESLEDLIAGIEVKK-

General information: TITO was launched using: RESULT: Template: 3EUP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 746 -49391 -66.21 -255.91 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : -66.21 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.446

(partial model without unconserved sides chains): PDB file : Tito_3EUP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EUP-query.scw PDB file : Tito_Scwrl_3EUP.pdb: