Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQEQIEKKKEEKQKAEKAALEKK 4TN5 Chain:B ((5-17)) ----VAVTACVSGVAHT-----------------------------------------------

General information: TITO was launched using: RESULT: Template: 4TN5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 10 -750 -75.00 -57.69 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain B : 0.71 3D Compatibility (PKB) : -75.00 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 1.257

(partial model without unconserved sides chains): PDB file : Tito_4TN5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TN5-query.scw PDB file : Tito_Scwrl_4TN5.pdb: