Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVFCFRLDKKEESAMAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDE-TGKQVVRTALNSGINLIDTAYAYGNGRSEELIGEVLKE-KEYDRSRVVIATKAAHVPNKGRTFDNSPEFLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTLEQLKEANA----DGY--VDVVEDKYSLIHRQA-EKELFPYLEKNKISFVPYFPLASGLLTGKYELGEEKQFGEGDPRKRNPDF-------QGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRVSVVIPGAKRPEQVSDNVQALDL--HLSNEDYQTIDEAFRGF 1QRQ Chain:C ((2-322)) --------------QFYRNLGKSGLRVSCLGLGTWVTFGG-----QITDEMAEHLMTLAYDNGINLFDTAEVYAAGKAEVVLGNIIKKKGWR-RSSLVITTKIFWGGKAETERGLSRKHIIEGLKASLERLQLEYVDVVFANRPDPNTPMEETVRAMTHVINQGMAMYWGTSRWSSMEIMEAYSVARQFNLIPPICEQAEYHMFQREKVEVQLPELFHKIGVGAMTWSPLACGIVSGKYDSGIPP---Y--SRASLKGYQWLKDKILSEEGRRQQAKLKELQAIAERLGCTLPQLAIAWCLRNEGVSSVLLGASNAEQLMENIGAIQVLPKLSSSIVHEIDSILGN-

General information: TITO was launched using: RESULT: Template: 1QRQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1760 24180 13.74 79.80 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain C : 0.72 3D Compatibility (PKB) : 13.74 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.578

(partial model without unconserved sides chains): PDB file : Tito_1QRQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1QRQ-query.scw PDB file : Tito_Scwrl_1QRQ.pdb: