Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSIMVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIANVAGACEEDYVEVCSKIGEAPNV---KAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKVSSVPVYVKLSPNV--TDIVPIAQAIEA-GGADGFTMINTL-LGMRIDLKTRKPILA--NQTGGLSGPAIKPVAIRLIHQVASIS-SLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTD-PYICPKLIKELPVRMSELGIESLERLIKEVREERER 3TQ0 Chain:B ((34-344)) --SMSLQVNLLNNTFANPFMNAAGVMCTTTEELVAMTESASGSLVSKSCTPALREGNPTPRYQALPLGSINSMGLPNNGFDFYLAYAAEQHD-YGKKPLFLSMSGLSMRENVEMCKRLAAV-ATEKGVILELNLSCPNVPGK-PQVAYDFDAMRQCLTAVSEVYPHSFGVKMPPYFDFAHFDAAAEILNEFPKVQFITCINSIGNGLVIDAETESVVIKPKQGFGGLGGRYVLPTALANINAFYRRCPGKLIFGCGGVYTGEDAFLHVLAGASMVQVGTALQEEGPSIFERLTSELLGVMAKKRYQTLDEFRGKVR-----

General information: TITO was launched using: RESULT: Template: 3TQ0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1947 -75202 -38.62 -250.67 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.74 3D Compatibility (PKB) : -38.62 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.461

(partial model without unconserved sides chains): PDB file : Tito_3TQ0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TQ0-query.scw PDB file : Tito_Scwrl_3TQ0.pdb: