Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVITPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKLGAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQDDVGTMFEKLSLLGRKLLLETLPNILDGQ-KPVPQDESEATFSPNITREQEAIDWHKTAEEIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQTVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK 3TQQ Chain:A ((2-310)) SLKIVFAGTPQFAVPTLRALIDSSHRVLAVYTQPD----------ESPVKEIARQNEIPIIQPFSLRDEVEQEKLIAMNADVMVVVAYGLILPKKALNAFRLGCVNVHASLLPRWRGAAPIQRAILAGDRETGISIMQMNEGLDTGDVLAKSACVISSEDTAADLHDRLSLIGADLLLESLAKLEKGDIKLEKQDEASATYASKIQKQEALIDWRKSAVEIARQVRAFNPTPIAFTYFEGQPMRIWRATVVDEKTDFEPGVLVDADKKGISIAAGSG-ILRLHQLQLPGKRVCSAGDFINAHGDKLIPGK---

General information: TITO was launched using: RESULT: Template: 3TQQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1610 -127479 -79.18 -427.78 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -79.18 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.539

(partial model without unconserved sides chains): PDB file : Tito_3TQQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TQQ-query.scw PDB file : Tito_Scwrl_3TQQ.pdb: