Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MARMAKVVELPSIAKIDKSKGKISAQLTQALREAVQSGDLQPGDPLPSSRELALTLGVARGTIIEAYDQLLAEGVFDSQPRTGTYVSHALVKTSAIQHSSKENKPNSISLNKSARSYAKVLDEFKPLPHVPFAVSVPIARTQPSDIWRKFGNKFRSRGAGVPSGYGEPQGVLSLRIAIADYVRRSRSVHCEPEQIVITPSIQQSLYICNQILFEAKDEVWVEDPAYRGTTAIFEHTVQNIRMVRVPVDEEGIQIETGIKL-S-KQASAAFVTPSHQYPLGMPMSLARRTALLAWAKQQNAWIIEDDYDSELRYNGQPFPSLQGMA---P-DQAIYLGTFSKVLFPSLRLGYAVLPKELVAPFCGLRVLIDRHPPAADQHVLAAFIQEGYLERHIRRTRNVYAEVREYIIGLIEKYIPQELAWLQLGDQGMHMVLWLAQHINDIDVASSAVDAGIAIKAISPTFSKERRRSGLIVGLGDFEPELMQQAIKKLSKIIQQHAKSM 2ZYJ Chain:A ((31-397)) -----------------------------------------------------------------------------------------------------------------------------RPGILSFAGGLPAPELFPKEEAAEAAARILREKGEVALQYSPTEGYAPLRAFVAEWI----G--VRPEEVLITTGSQQALDLVGKVFLDEGSPVLLEAPSYMGAIQAFRLQGP--RFLTVPAGEEGPDLDALEEVLKRERPRFLYLIPSFQNPTGGLTPLPARKRLLQMVMERGLVVVEDDAYRELYFGEARLPSLFELAREAGYPGVIYLGSFSKVLSPGLRVAFAVAHPEALQKLVQAKQGADLHTPMLNQMLVHELLKEGF-SERLERVRRVYREKAQAMLHALDREVPKEVRYT-RPKGGMFVWMELPKGLSAEGLFRRALEENVAFVPGGPFFANGGGENTLRLSYATLDREGIAEGVRRLGRALKGLLALV

General information: TITO was launched using: RESULT: Template: 2ZYJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2053 -34603 -16.85 -95.85 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -16.85 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.496

(partial model without unconserved sides chains): PDB file : Tito_2ZYJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZYJ-query.scw PDB file : Tito_Scwrl_2ZYJ.pdb: