Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVGENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPARSAVEVARLPRDVKVEIEVIAEIG 2CVL Chain:B ((2-124)) --EAVKTDRAPAAIGPYAQAVKAGGFVFVSGQIPLAPDGSL-VEGDIRVQTERVMENLKAVLEAAGSGLSRVVQTTCFLADMEDFPGFNEVYARYFTPPYPARATVAVKALPRGVRVEVACVALAE

General information: TITO was launched using: RESULT: Template: 2CVL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 615 -55700 -90.57 -452.85 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : -90.57 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.492

(partial model without unconserved sides chains): PDB file : Tito_2CVL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CVL-query.scw PDB file : Tito_Scwrl_2CVL.pdb: