Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---MTQVDIAALSPQVVWQHFQTLCTIPRPSKHEQQLREFLQNWAESRNL----ETYVDEVGNLIIRKNATPGKKHVSGVILQGHLDMVTQANTGTVHDFFKDPIRP--VLEEGWLIAKDTTLGADNGIGVALALAVLDSNDIHGPIEVLLTVDEEAGMSGARLLETGVLKGKWLFNIDTEEWGELYLGCAGSIDVE-VEQPLNYEPIPENLNIVNIQVAGLKGGHSGVDIHLGRGNANVILARFLNQHLASLGGHLVEFTGGTARNALPREAVATIAISPNQLSSLEKLLAEYQAAWKEQLKGIDDNLQLSIQSTGAKVTEVINQQQQNEWLQALATSPYGVASMSQVLPDVVETSNNIGV---VRLNREGGKAILMVRSMVNQEAQDFAEKIQAHFSQFNIGSTLTPLVS--GWTPNPDSAALKCLQQAYQNAFNIEPNLKVIHAGLECGIIAEHYPHLQ----MVSFGPDIQGAHAPGERVKVDTVEKCWKLLVTALASVE---- 5K8P Chain:A ((5-425)) NDVAKVMKTLDGMREGLIQTAVELGSIEAPTGREGAAGDYVYEWMARNGFGPERVGVFDDRFNVVGRLRGTGGG---ASLSFNSHLDTIMAREDTARFADANDRIYHEAWHEEGRIY------------GYSVV-------NCKGPMACWLIAAKALKEAGAALKGDVVLTAV-----------------CGEIDCEPVDEFQGHDYLAEDIGARYAISHGAISDYALVAEATNFKPAWVEAGKVFLKVTVFAGP-----SRYTPYVPRPVAALD----SPNAIVRMAKLVEALEE-WADNYEKRY-------------------------------TREYGG---GTVVPKVAIGAIRGGVPYKIYAFPELCSIYMDIRLNPDTNPLVVQREVEAVVSKLGLKAEVKPFLFRRGYEAQGIEPLQNALEVAHREVVG-RPTERP--GSPECSMWRDTNPYNELGIPSLTYGCG-GGAGGGNTYFLVDDMLKAAKVYAMTAMDLCNRTP

General information: TITO was launched using: RESULT: Template: 5K8P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2292 45302 19.77 113.82 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 19.77 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.331

(partial model without unconserved sides chains): PDB file : Tito_5K8P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5K8P-query.scw PDB file : Tito_Scwrl_5K8P.pdb: