Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSGSTITPWVVGNWKMNPMRANANQLIEEFKQ--LLQQNQIADENCHVGVAPVSIALTT--VQAQLQDAARTVYTVAQDVSRVAGTGAYTGEVSAELLKDSQINFVLVGHSERRDIFGDNVEILKAKLQNALNAGMTVIYCVGESLEQREQGQAEQVVLQQICDIAPVVTAEQWQNQVVIAYEPIWAIGTGKTASPQDAQAMHAKIREGLCQLTPA--GSNIAILYGGSVKAENAVELAACPDINGALVGGASLNAASFYQIVQAFAQSK 5EYW Chain:A ((8-247)) --------FVGGNWKMNGDKAAIDGIISFMKTGPLSPNTEVV-----VGCPQCYLMYTREHMPANIGIAAQNCYKVAK--------GAFTGEISPAMVKDCGCEWVILGHSERRNVFGEPDQLISEKVGHALEAGLKVIPCIGEKLEDREGNRTQEVVFAQMKALLPNIS--DW-SRVVLAYEPVWAIGTGKTASPEQAQEVHADLRQWLRDNVNAEVAESTRIIYGGSVSAGNCQELAKKGDIDGFLVGGAALK-PDFVQIINA-----

General information: TITO was launched using: RESULT: Template: 5EYW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1258 -39361 -31.29 -168.21 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -31.29 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.584

(partial model without unconserved sides chains): PDB file : Tito_5EYW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EYW-query.scw PDB file : Tito_Scwrl_5EYW.pdb: