Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALLPILSFPDPRLRTIAKPVEEVTDEIRQLAADMFETMYAAPGIGLAASQVDRHIQLIVMDL-----SESKDEPMVFINPKVTPLTEETQPYEEGCLSVPQIYDKVDRPSRVKIEAINLEGQAFEIEADGLLAVCIQHEMDHLNGKLFVDYLSPLKRQRVREKVEKIVRQREREKVAVKR 4E9A Chain:A ((1-163)) -ALLEIIHYPSKILRTISKEVVSFDAKLHQQLDDMYETMIASEGIGLAAIQVGLPLRMLIINLPQEDGVQHKEDCLEIINPKFIE-TGGSMMYKEGCLSVPGFYEEVERFEKVKIEYQNRFAEVKVLEASELLAVAIQHEIDHLNGVLFVDKLSILKRKKFEKEL----------------

General information: TITO was launched using: RESULT: Template: 4E9A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 764 -32441 -42.46 -205.32 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -42.46 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.570

(partial model without unconserved sides chains): PDB file : Tito_4E9A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4E9A-query.scw PDB file : Tito_Scwrl_4E9A.pdb: