Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MREIILISFLGPDQPNQFTRLMQVLSVHSLQILDVGQAVIHNQLTLGIVVASENETA-TALAMKEILILAHDIGLTVRFKPISGAEYDQWVSEGGRTRYIVTALAPELTAAHLQAVTQIVSSQGFNIETVTRLSGRVDLEKDSTLPRRACVQFGLSSGPTLDAQAMRAACLLLSSELNIDVAVQEDNAYRRNRRLVCFDMDSTLIEQEVIDELALEAGVGEQVAEITERAMQGELDFQQSFRARVALLKGLDASVLPKIAERLTITEGAERLISTLKVLGYKTAILSGGFQYFAEYLQAKLGIDEVHANVLDVQDGVVTGEVKGVIVDGARKAELLRELANKLGISLEQAMAVGDGANDLPMLAIAGLGVAYRAKPLVRQNANQAISSVGLDGVLYLLGMHDKDLSRA 5IS2 Chain:A ((2-391)) -KVSVLITVTGVDQPGVTATLFEVLSRHGVELLNVEQVVIRHRLTLGVLVCCPADVADGPALRHDVEAAIRKVGLDVSIERSDDV-----PIIREPSTHTIFVLGRPITAAAFGAVAREVAALGVNIDLIRGVSD----------YPVIGLELRVSVPPG-ADGALRTALNRVSSEEHVDVAVEDYTLERRAKRLIVFDVDSTLVQGEVIEMLAAKAGAEGQVA-ITDAAMRGELDFAQSLQQRVATLAGLPATVIDEVAGQLELMPGARTTLRTLRRLGYACGVVSGGFRRIIEPLAEELMLDYVAANELEIVDGTLTGRVVGPIIDRAGKATALREFAQRAGVPMAQTVAVGDGANDIDMLAAAGLGIAFNAKPALREVADASLSHPYLDTVLFLLGVTRGEIEA-

General information: TITO was launched using: RESULT: Template: 5IS2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2150 -48099 -22.37 -123.97 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -22.37 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.566

(partial model without unconserved sides chains): PDB file : Tito_5IS2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IS2-query.scw PDB file : Tito_Scwrl_5IS2.pdb: