Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKMNKQPQNSALVVVDVQNGFTP-GGNLAVADADTIIPTINQLAGCFE--NVVLTQDWHPDNHISFAANHPGKQPFETIELDYGSQVLWPKHCIQGTHDAEFHPDLNIPTAQLIIRKGFHAHIDSYSAFMEADHTTMTGLTGYLKERGIDTVYVVGIATDFCVAWTALDAVKQGFKTLVIEDACKGIDLNGSLEQAWQTMQQQGVIRIQSTDLLNEC 3R2J Chain:B ((28-223)) --VTVSSTTDVLIIADMQVDFLAPGGSLHVKGGEALLDGINAVSSQLPFRYQVATQDWHPENHCSFV----------------THGGPWPPHCVQGSAGAQLHAGLHTQRINAVIRKGVTQQADSYSAFVEDN-GVSTGLAGLLHSIGARRVFVCGVAYDFCVFFTAMDARKNGFSVVLLEDLTAAVDDAA-WSARTAELKDAGVVLLKSSALVAE-

General information: TITO was launched using: RESULT: Template: 3R2J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1105 -34546 -31.26 -178.99 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -31.26 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.509

(partial model without unconserved sides chains): PDB file : Tito_3R2J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3R2J-query.scw PDB file : Tito_Scwrl_3R2J.pdb: