Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MREKLKGGNIMKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQSTEQTTTEMKQSVETSASSITES-SSEEVKNTLWDTNKASKLETFVT-QWGKTLGQEYKSYNLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSD------VETGEYLGQHVYFFGFQNGQPKVYLT-QQNQGNENNYLYFNETQNQQLKQGFSDI---VNGQTPQTPVVEESTQQ-----ATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNEYMKDLMQDENG--SYYSINIVTDDDGNTT------MAGGKQFRVYTDGRISQRIGMTNNFQMIK----- 3NOY Chain:A ((10-358)) MIQKRKTRQ---------IRVGNVKIGGDA-----PIVVQSMTS-----TKTHDVEATLNQIKRLYEAGCEIVRVAVPHKEDVEALEEIVKKSPMPVIADIHFAPSYAFLSMEKGV-HGIRINPGNIGKEEIVREIVEEAKRRGVAVRIGVN-SGSLEKDLLEKYGYPSAEALAESALRWSEKFEKWGFTN--YKVSIKGSDVLQNVRANLIFAERTDVPLHIGITEAGMGTKGIIKSSVGIGILLYMGIGDTVRVSLTDDPVVEVETAYEILKSLGLRRRGVEIVACPTCGRIEVDLPKVVKEVQEKLSGVKTPLKVAVMGCVVNAIGEAREADIGLACGRGFAWLFKHGKPIKKVDESEMVDELLKEIQN

General information: TITO was launched using: RESULT: Template: 3NOY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1723 83885 48.69 262.96 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 48.69 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.123

(partial model without unconserved sides chains): PDB file : Tito_3NOY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3NOY-query.scw PDB file : Tito_Scwrl_3NOY.pdb: