Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAESTTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEYAPKAVENF--MTHAKDG--------YYNGLTFHRVIKDFMIQGGD-PKGDGTGGESIWGEGFETEINNHLYNIRGALSMARSQDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGMDVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK 1E8K Chain:A ((18-165)) ------------------------------------------------------------------------------GRIVMELYDDVVPKTAGNFRALCTGENGIGKSGKPLHFKGSKFHRIIPNFMIQGGDFTRGNGTGGESIYGEKFPDENFKEKHTGPGVLSMANA-GPNTNGSQFFLCT---------VKTEW---------------LDGKHVVFGRVVEGLDVVKAVES-NGSQSGKPVKDCMI------------

General information: TITO was launched using: RESULT: Template: 1E8K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 703 31073 44.20 226.81 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 44.20 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.474

(partial model without unconserved sides chains): PDB file : Tito_1E8K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1E8K-query.scw PDB file : Tito_Scwrl_1E8K.pdb: