Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFFQGHFPEEPVMPGVLIVEAMAQAGA-VALLSLEQF-----KGKTAYFGGLDKAKFRKKVTPGDTLYLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG 3B7J Chain:B ((14-156)) --IEHILQILPHRYPMLLVDRITELQANQKIVAYKNITFNEDVFNGHFPNKPIFPGVLIVEGMAQSGGFLAFTSLWGFDPEIAKTKIVYFMTIDKVKFRIPVTPGDRLEYHLEVLKHKGMIWQVGGTAQVDGKVVAEAELKAMIA

General information: TITO was launched using: RESULT: Template: 3B7J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 617 -43190 -70.00 -315.25 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.84 3D Compatibility (PKB) : -70.00 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.595

(partial model without unconserved sides chains): PDB file : Tito_3B7J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3B7J-query.scw PDB file : Tito_Scwrl_3B7J.pdb: