Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVITPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKLGAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQDDVGTMFEKLSLLGRKLLLETLPNILDGQ-KPVPQDESEATFSPNITREQEAIDWHKTAEEIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQTVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK 3Q0I Chain:A ((7-317)) SLRIVFAGTPDFAARHLAALLSSEHEIIAVYTQPE---------TASPVKTLALEHNVPVYQPENFKSDESKQQLAALNADLMVVVAYGLLLPKVVLDTPKLGCINVHGSILPRWRGAAPIQRSIWAGDSETGVTIMQMDVGLDTGDMLKIATLPIEASDTSASMYDKLAELGPQALLECLQDIAQGTAVAVKQDDGLANYAHKLSKEEARINWSDAATHIERCIRAFNPWPMSHFEVAENSIKVWQARVETRAVTQTPGTIIQADKSGIYVATGQD-VLVLESLQIPGKKALPVQDILNARADWFSVGSQL-

General information: TITO was launched using: RESULT: Template: 3Q0I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1631 -130103 -79.77 -432.24 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -79.77 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.523

(partial model without unconserved sides chains): PDB file : Tito_3Q0I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3Q0I-query.scw PDB file : Tito_Scwrl_3Q0I.pdb: