Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVYILNSTEYTLDRIDRKILSALRRNGRLTVAQLAEEVGLSSSPCWTRLKRLESLKIIEGYTVNVNPKAIGIHELFFIEITLERHDDEMLENFSEALADIPEVVEAHLVTGDYDYLVKVAVKDAEHYERFLRKKLYSIKGIRHTRSTFALRPLKSANTADLMLIE 2P5V Chain:G ((3-160)) ------MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLKQLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKA-KDAREDFAASVRKWPEVLSCFALTGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLL

General information: TITO was launched using: RESULT: Template: 2P5V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 534 -92237 -172.73 -583.78 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain G : 0.79 3D Compatibility (PKB) : -172.73 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.550

(partial model without unconserved sides chains): PDB file : Tito_2P5V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2P5V-query.scw PDB file : Tito_Scwrl_2P5V.pdb: