Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLIIPAIDLKDGKCVRLKQGRMEDDTVFSDDLVATAQHWVNEGARRLHLVDLNGAFAGTPIHKPVVEAIAKAQPELPIQIGGGIRSLETIEHYLEAGVTFVIIGTKAVQEPEFVEEACKSFAGHIIVGIDAMNGMVATDGWANVTDVKATDLAKRFADAGVSSIVYTDIARDGMMQGVNVEQTVNLAQYSGLPVIASGGVTNLDDVRNLKGQL------GILGAITGRAIYEGTLNLREAQLLLDENRL 2CFF Chain:A ((1-240)) MLVVPAIDLFRGKVARMIKGRKENTIFYEKDPVELVEKLIEEGFTLIHVVDLSNAIENSGENLPVLEKLSE-FA-EHIQIGGGIRSLDYAEKLRKLGYRRQIVSSKVLEDPSFLKSLRE-IDVEPVFSLVTRGGRVAFKGWLAEEEIDPVSLLKRLKEYGLEEIVHTEIEKDGTLQEHDFSLTKKIAIEAEVKVLAAGGISSENSLKTAQ-KVHTETNGLLKGVIVGRAFLEGILTVEVMKRYA-----

General information: TITO was launched using: RESULT: Template: 2CFF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1256 -139653 -111.19 -596.81 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -111.19 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.622

(partial model without unconserved sides chains): PDB file : Tito_2CFF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CFF-query.scw PDB file : Tito_Scwrl_2CFF.pdb: