Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNQKYQIKLSQLIKGCFIYAIANYALAQPIVVEGVVPNEASKQAILMKMQSVYGADQVVDKIQVRPVAAPNGWSDSVTRVITPDLKKVSQGKLSVNGTRFELTGKMLNPTEIQPTIQSFQSLVQPPYQLFSQLSVNQAEQKIIDDALKNRIIEFESGSAVLTEAGQKILDEMAVALNKVGGK--KVKIVGHTDSSGDATKNLKLSQDRALAVKNYLISKNIPADHLSTEGLGSSKPVADNTSAEGRKK---------NRRIEFTVL 4ERH Chain:B ((19-137)) -------------------------------------------------------------------------------------------------------------------------------------------------TLKSDVL-FNFNKSTLKPEGQQALDQLYSQLSNLDPKDGSVVVLGFTDRIGSDAYNQGLSEKRAQSVVDYLISKGIPSDKISARGMGESNPVTGNTCDNVKPRAALIDCLAPDRRVEIEV-

General information: TITO was launched using: RESULT: Template: 4ERH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 349 14736 42.22 136.44 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.62 3D Compatibility (PKB) : 42.22 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.618

(partial model without unconserved sides chains): PDB file : Tito_4ERH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ERH-query.scw PDB file : Tito_Scwrl_4ERH.pdb: