Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAKHWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQGEKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKE-LGLNTRPVLVGPFTFLQLSDFEEGVKADDFVDSFVAAYQEVFAKLADLGATRIQLDEAALVKDLTAEEKALFLNLYNKLLADKKGLEVLFQTYFGDVRDVYADLVNLPVDAIGLDFVEGK-KTLELVKGGFPADKTLYVGIVNGKNIWRNNYEKSLTVLEQI-P-AENIVLTSSCSLLHVPFTTANE-EFEPALLNHFAFAVEKLDEIRDLDAIRNGQGS--E-A----LAANKELFATERVGENAELRARIAGLTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKEYDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGMRGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKDSTLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVHSTVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVGPGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVEAAKAAREKL 3T0C Chain:A ((36-777)) TKVSSLGYPRLGENREWKKLIEAYWAGKVSKNDLFAGAKELRLDFLKKQLNAGLDLIPVGDFSLYDHILDLSVQFNIIPKRFAKEPI-DIDLYFAIARGN-E----NVASSMKKWFNTNYHYIVPEWSK-QRPKLNNNRLLDLYLEAREVVGDKAKPVITGPITYVALSTGVE--DFTAAVKSLLPLYKQVFTELVKAGASYIQVDEPIFVTDEGKDYLQAAKAVYAYFAKEVPDAKFIFQTYFEGLIDS-QVLSQLPVDAFGLDFVYGLEENLEAIKTGAFKGKEIFAGVIDGRNIWSSDFVKTSALLETIEEQSAALTIQPSCSLLHVPVTTKNETDLDPVLRNGLAFADEKLTEVKRLAEHLDGREDPAYDLHIAHFDAL-----QAADFRN--VKLE--DLSRVATKRPSDFAKRRDIQQEKLHLPLLPTTTIGSFPQS--IRRTR-AW--GDISDAEYKQFIQAEIERWIRIQEDLDLDVLVHGEFERVDMVEFFGQKLAGFTTTKFGWVQSYGSRAVKPPIIYGDVQHLEPITVEETVYAQSLTDRPVKGMLTGPITITNWSFERTDIPRDQLFNQIGLAIKDEIKLLENAGIAIIQVDEAALREGLPLRKSKQK-AYLDDAVHAFHIATSSVKDETQIHTHMCYSKFDEIIDAIRALDADVISIETSRSHGDIIESFETAVYPLGIGLGVYDIHSPRVPTKEEVVANIERPLRQLSPTQFWVNPDCGLKTRQEPETIAALK-LVAATKEVRQKL

General information: TITO was launched using: RESULT: Template: 3T0C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 4172 46127 11.06 63.80 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : 11.06 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.487

(partial model without unconserved sides chains): PDB file : Tito_3T0C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3T0C-query.scw PDB file : Tito_Scwrl_3T0C.pdb: