Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRPFLLLEVLEALQVAIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFGEAMAILAGDALFLDPYALIAQADL-PSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ--HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVLDVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQVPFETESIVSVVESLRING 3M0G Chain:B ((9-272)) ----EVQDAVETAMAAAIGRL-PAGDLRDAMAYAA-QGGKRLRAFLAIESAAIHGISMAQA-MPAALAVEALHAYSLVHDDMPCMDNDDLRRGLPTVHKKWDDATAVLAGDALQTLAFELCTDPVLGSAENRVALVAALAQASGAEGMVYGQALDIAAETAAVPLTLDEIIRLQAGKTGALISFAAQAGAILAGADR---GPLTAYATALGLAFQIADDILDV------------------KATFVSLLGLAGAKSRAADLVAEAEAALAPYGE--------------------

General information: TITO was launched using: RESULT: Template: 3M0G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1209 -96163 -79.54 -395.73 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.77 3D Compatibility (PKB) : -79.54 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.532

(partial model without unconserved sides chains): PDB file : Tito_3M0G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3M0G-query.scw PDB file : Tito_Scwrl_3M0G.pdb: