Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQLTLVRHGEAAPPVN---GNDIKRPLTARGHAQAEQTATFLKDIVKPNIFVVSPLLRAQETLAHIQTYFKDVPVLLCDKI-----------------------------------KPDDDAKE-----AIEWLSQI----PYESIVVVCHMNVVGHIAELLTHETF------NPFALAEARIYDQAVIANGLSTQKNSFIPTI 1H2E Chain:A ((2-199)) TTLYLTRHGETKWNVERRMQGWQDSPLTEKGRQDAMRLGKRLEAVE-LAAIYTSTSGRALETAEIVRGGRL-IPIYQDERLREIHLGDWEGKTHDEIRQMDPIAFDHFWQAPHLYAPQRGERFCDVQQRALEAVQSIVDRHEGETVLIVTHGVVLKTLMAAFKDTPLDHLWSPPYMYGTSVTIIEVDG---GTFHVAVEGDVS-

General information: TITO was launched using: RESULT: Template: 1H2E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 607 9249 15.24 63.79 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 15.24 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.414

(partial model without unconserved sides chains): PDB file : Tito_1H2E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1H2E-query.scw PDB file : Tito_Scwrl_1H2E.pdb: